Development of a solid-phase radioimmunoassay for serum cortisol that effectively inactivates interfering binding proteins.
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May simplify lab cortisol measurement; leaves open validation before routine clinical use.
We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.
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Brock et al. (1978) studied this question.
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