July 23, 2013The Journal of PhysiologyOpen Access

The working stroke of the myosin II motor in muscle is not tightly coupled to release of orthophosphate from its active site

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Population

Skinned muscle fibres from rabbit psoas

Design

Preclinical

Authors

MCMarco CaremaniSynlab Czech (Czechia)LMLuca MelliRoche (Switzerland)MDMario DolfiUniversity of Florence

Discussion

Member takes

Implication

Challenges prior tight-coupling models of myosin kinetics; hypothesis-generating for phosphate release timing in cardiac muscle.

Structured PICO

Population

Skinned muscle fibres from rabbit psoas

Intervention

Stepwise drops in force on an otherwise isometric contraction using nanometre-microsecond mechanics

Outcome

Isotonic velocity transient and rate of regeneration of the working strokesurrogate

The release of inorganic phosphate from the catalytic site of a myosin motor can occur at any stage of the working stroke, challenging previous models of tight coupling.

Cite This Study

Caremani et al. (2013) studied this question.

synapsesocial.com/papers/6a87c6a4350e059174ad31cb https://doi.org/10.1113/jphysiol.2013.257410

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The working stroke of the myosin II motor in muscle is not tightly coupled to release of orthophosphate from its active site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The working stroke of the myosin II motor in muscle is not tightly coupled to release of orthophosphate from its active site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider