Molecular epidemiology of enterovirus outbreaks in Canada during 1991–1992: Identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing

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Population

Enteroviral isolates associated with two outbreaks in Canada during 1991-1992

Comparison

Direct sequencing of amplicons derived from the… vs Prototype strains

Design

Other

Authors

Discussion

May aid rapid outbreak strain identification via 5' NTR sequencing; leaves open prospective validation and clinical impact.

Population

Enteroviral isolates (60 echovirus 30 isolates and recent coxsackievirus B1 isolates) associated with two outbreaks in Canada during 1991-1992

Intervention

Direct sequencing of amplicons derived from the 5' nontranslated region (NTR) of the viral genome

Comparator

Prototype strains

Outcome

Sequence identity and relatedness of isolates

Variability in the 5' NTR nucleotide sequence can be used to rapidly identify enteroviral strains associated with specific outbreaks.

Drebot et al. (1994) studied this question.

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