What question did this study set out to answer?

To determine how deletion of the angiotensin II AT2 receptor alters hypothalamic AT1 receptor expression and the activity of the hypothalamic-pituitary-adrenal axis.

January 1, 2002Neuroendocrinology

Increased Angiotensin II AT1 Receptor Expression in Paraventricular Nucleus and Hypothalamic-Pituitary-Adrenal Axis Stimulation in AT2 Receptor Gene Disrupted Mice

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Population

Angiotensin II AT2 receptor gene-disrupted mice and wild-type controls; wild-type C57 BL/6J mice

Comparison

AT2 receptor gene disruption; Candesartan for 2… vs Wild-type controls

Design

Preclinical

Follow-up

2 weeks

Authors

IAInés ArmandoGeorge Washington University JTJosé A. TerrónInstituto Politécnico Nacional AFAlicia Falcón‐NeriUniversidad Nacional Autónoma de México

Discussion

Member takes

Implication

AT2 disruption alters HPA signaling in mice; hypothesis-generating for receptor cross-talk but should not yet change practice.

Key Points

To determine how deletion of the angiotensin II AT2 receptor alters hypothalamic AT1 receptor expression and the activity of the hypothalamic-pituitary-adrenal axis.
Assessed hypothalamic AT1 receptor binding and mRNA, adrenal catecholamines, tyrosine hydroxylase mRNA, and plasma hormones in AT2 receptor knockout and wild-type mice.
Treated wild-type C57 BL/6J mice with the AT1 receptor antagonist candesartan for two weeks to determine the specific role of AT1 signaling on adrenal function.
Mice lacking the AT2 receptor showed elevated AT1 receptor binding in the paraventricular nucleus and median eminence, higher plasma ACTH and corticosterone, and reduced adrenal aldosterone.
AT2 gene disruption increased adrenal tyrosine hydroxylase mRNA alongside elevated dopamine, norepinephrine, and epinephrine levels, whereas candesartan treatment in wild-type mice significantly reduced these markers.

Structured PICO

Population

Angiotensin II AT2 receptor gene-disrupted mice and wild-type controls; wild-type C57 BL/6J mice

Intervention

AT2 receptor gene disruption; Candesartan for 2 weeks (in wild-type mice)

Comparator

Wild-type controls

Outcome

Hypothalamic AT1 receptor binding and mRNA, adrenal catecholamines, adrenal tyrosine hydroxylase mRNA, and pituitary and adrenal hormonessurrogate

AT2 receptor gene disruption leads to increased AT1 receptor expression in the hypothalamic-pituitary-adrenal axis, providing a mechanism for increased stress sensitivity and supporting AT1/AT2 receptor cross-talk.

Cite This Study

Armando et al. (2002) studied this question.

synapsesocial.com/papers/6a87d6a76c84a38dade956d4 https://doi.org/10.1159/000064525

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Increased Angiotensin II AT1 Receptor Expression in Paraventricular Nucleus and Hypothalamic-Pituitary-Adrenal Axis Stimulation in AT2 Receptor Gene Disrupted Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Increased Angiotensin II AT1 Receptor Expression in Paraventricular Nucleus and Hypothalamic-Pituitary-Adrenal Axis Stimulation in AT2 Receptor Gene Disrupted Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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