What question did this study set out to answer?

To determine and compare the optimal DNA binding target sequences for MEF2 proteins in muscle and brain tissues to understand how tissue-specific transcriptional regulation occurs.

October 1, 1995Journal of Biological ChemistryOpen Access

Determination of the Consensus Binding Site for MEF2 Expressed in Muscle and Brain Reveals Tissue-specific Sequence Constraints

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To determine and compare the optimal DNA binding target sequences for MEF2 proteins in muscle and brain tissues to understand how tissue-specific transcriptional regulation occurs.
Selected optimal DNA target sequences from a degenerate oligonucleotide library using anti-MEF2A antibodies.
Tested and compared binding site selection using protein extracts prepared from skeletal muscle, cardiac muscle, and brain.
Identified a shared core consensus binding motif, 5'-CT(A/t)(a/t)AAATAG-3', across skeletal muscle, cardiac muscle, and brain extracts.
Demonstrated that brain-derived MEF2 requires an extended consensus sequence (5'-TGTTACT(A/t)(a/t)AAATAGA(A/t)-3') with additional flanking sequence constraints absent in muscle extracts.

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Implication

In vitro analysis reveals distinct DNA binding constraints for MEF2 in brain versus muscle extracts, indicating how identical transcription factors achieve tissue-specific gene regulation.

Cite This Study

Andrés et al. (1995) studied this question.

synapsesocial.com/papers/6a87e1b424ca9e5e630162dc https://doi.org/10.1074/jbc.270.40.23246

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Implication

In vitro analysis reveals distinct DNA binding constraints for MEF2 in brain versus muscle extracts, indicating how identical transcription factors achieve tissue-specific gene regulation.

Determination of the Consensus Binding Site for MEF2 Expressed in Muscle and Brain Reveals Tissue-specific Sequence Constraints

Key Points

Discussion

Implication

Cite This Study

Determination of the Consensus Binding Site for MEF2 Expressed in Muscle and Brain Reveals Tissue-specific Sequence Constraints

Key Points

Discussion

Implication

Cite This Study

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