Why the study?
Does an immunoradiometric assay accurately measure plasma levels of active renin compared to plasma renin activity?
Does an immunoradiometric assay accurately measure plasma levels of active renin compared to plasma renin activity?
The study validates an immunoradiometric assay as a reliable tool for measuring active renin, facilitating research on renin inhibitors and hypertension.
Answers are grounded in this paper and cite their sources.
Supports research on renin inhibitors in hypertension; leaves open routine clinical adoption pending further validation.
An immunoradiometric assay (IRMA) for active renin in human plasma was analytically and clinically validated. Analytical validation established 1) precision, 2) recovery, 3) linearity, 4) cross-reactivity, 5) sample stability, and 6) the validity and specificity of the 125I-labeled anti-renin monoclonal in the Diagnostics Pasteur immunoradiometric renin kit. Clinical validation included 1) establishing normal reference range for renin, 2) comparing plasma renin activity (PRA) results to immunoreactive renin levels in subjects on Upjohn research protocols, and 3) comparing the renin responsiveness of sodium replete subjects to that of sodium deplete subjects prior to, during, and after infusion with Upjohn renin inhibitory peptide, ditekiren. This study was undertaken to demonstrate the research validity of an assay tool for the differentiation of enzymatically active renin from inactive renin or a form of prorenin.
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Wathen et al. (1991) studied this question.
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