What question did this study set out to answer?

Investigate whether engineered IgG and IgA monoclonal antibodies carrying the b12 variable region can prevent mucosal transmission and epithelial cell transfer of HIV-1.

September 1, 2007The Journal of ImmunologyOpen Access

Inhibition of HIV-1 Infectivity and Epithelial Cell Transfer by Human Monoclonal IgG and IgA Antibodies Carrying the b12 V Region

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Investigate whether engineered IgG and IgA monoclonal antibodies carrying the b12 variable region can prevent mucosal transmission and epithelial cell transfer of HIV-1.
Engineered recombinant human IgA2 monoclonal antibody forms (monomeric, dimeric, polymeric, and secretory) harboring the variable regions of the broadly neutralizing anti-gp120 antibody IgG1 b12.
Assessed antibody binding to gp120 and neutralization efficiency against CCR5- and CXCR4-tropic strains of HIV-1 in vitro.
Measured antibody-mediated inhibition of cell-free HIV-1 attachment, uptake, and transfer from human ME-180 cervical and Caco-2 colonic epithelial cell lines to human peripheral blood mononuclear cells.
All IgA2 variants of b12 recognized gp120 and successfully neutralized both CCR5- and CXCR4-tropic HIV-1 strains in vitro.
Both IgG1 b12 and IgA2 b12 significantly inhibited the transfer of cell-free HIV-1 from ME-180 cervical and Caco-2 colonic epithelial cells to human PBMCs.
Transfer inhibition resulted from the ability of b12 antibodies to block both viral attachment to and cellular uptake by the epithelial cells.

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Implication

In vitro study reveals that b12 IgG and IgA antibodies block HIV-1 transfer across human epithelial cells, suggesting a protective mechanism against mucosal viral transmission.

Cite This Study

Mantis et al. (2007) studied this question.

synapsesocial.com/papers/6a880a0e8e586ad1c8a536b8 https://doi.org/10.4049/jimmunol.179.5.3144

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Implication

In vitro study reveals that b12 IgG and IgA antibodies block HIV-1 transfer across human epithelial cells, suggesting a protective mechanism against mucosal viral transmission.

Inhibition of HIV-1 Infectivity and Epithelial Cell Transfer by Human Monoclonal IgG and IgA Antibodies Carrying the b12 V Region

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Discussion

Implication

Cite This Study

Inhibition of HIV-1 Infectivity and Epithelial Cell Transfer by Human Monoclonal IgG and IgA Antibodies Carrying the b12 V Region

Key Points

Discussion

Implication

Cite This Study

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