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In this paper, we describe the apparent production of enzymatically synthesized amylose in DMSO by means of the calcium alginate hydrogel beads/DMSO system as the reaction field of the phosphorylase-catalyzed enzymatic polymerization. When the calcium alginate hydrogel beads including glucose-1-phosphate, maltoheptaose, and phosphorylase were suspended in DMSO and the system was slowly stirred at 40°C for 12 h, the reaction proceeded to produce amylose, which eluted to the DMSO solution. The obtained amylose was purified by the treatment with ion-exchange resins, and its structure was confirmed by the 1H NMR spectrum. The time-course experiment in the present system revealed that the phosphorylase-catalyzed enzymatic polymerization was carried out for 15 min on the inside of the calcium alginate hydrogel beads and the produced amylose was gradually eluted to the surrounding DMSO solution. The comparison of the present system with the general enzymatic polymerization in aqueous buffer solution suggested that the yield and the degree of polymerization of amylose in the present system were comparable to those in aqueous buffer solution.
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Izawa et al. (2009) studied this question.
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