What question did this study set out to answer?

To investigate the phase partitioning of triphosphoinositide in the presence of divalent cations and characterize its binding to proteins to form phosphatido-peptide-like complexes.

October 1, 1965Biochemical JournalOpen Access

‘Phosphatido-peptide’-like complexes formed by the interaction of calcium triphosphoinositide with protein

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Authors

RDRMC DawsonConsiglio per la ricerca in agricoltura e l’analisi dell’economia agraria

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Implication

In vitro study demonstrates calcium triphosphoinositide binds proteins to form acid-soluble complexes, highlighting the chemical nature of tissue phosphatido-peptide assemblies.

Key Points

To investigate the phase partitioning of triphosphoinositide in the presence of divalent cations and characterize its binding to proteins to form phosphatido-peptide-like complexes.
Assayed triphosphoinositide partitioning between polar and non-polar phases in a chloroform-methanol-water system with added Ca(2+) or Mg(2+).
Measured protein-lipid binding at the solvent interface using serum albumin and evaluated complex dissociation using chelating agents EDTA and cyclohexane-1,2-diaminetetra-acetate.
Tested complex solubility and extraction behavior after homogenizing dicalcium triphosphoinositide with serum albumin and guinea-pig liver tissue in neutral versus acidified chloroform-methanol.
Adding 2 µg-atoms of Ca(2+) or Mg(2+) per µmole of triphosphoinositide shifted the lipid entirely into the lower non-polar phase; Ca(2+) affinity was 2–2.5 times higher than Mg(2+) and resisted equimolar chelator dissociation.
Dicalcium triphosphoinositide formed complexes with serum albumin and guinea-pig liver tissue that were insoluble in neutral chloroform-methanol (2:1, v/v) but dissolved completely upon adding 0.25% (v/v) concentrated hydrochloric acid.

Cite This Study

RMC Dawson (1965) studied this question.

synapsesocial.com/papers/6a8812a6ce59b4d55bcf07ab https://doi.org/10.1042/bj0970134

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Implication

In vitro study demonstrates calcium triphosphoinositide binds proteins to form acid-soluble complexes, highlighting the chemical nature of tissue phosphatido-peptide assemblies.

‘Phosphatido-peptide’-like complexes formed by the interaction of calcium triphosphoinositide with protein

Discussion

Member takes

Implication

Key Points

Cite This Study

‘Phosphatido-peptide’-like complexes formed by the interaction of calcium triphosphoinositide with protein

Discussion

Member takes

Implication

Key Points

Cite This Study

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