Key result
Baseline cTnI concentrations showed quantifiable differences between rat strains, sexes, and surgical alterations, with male SHR rats having higher levels (26 pg/ml) than females (10 pg/ml).
Observational (n=115)
Ultrasensitive immunoassays can detect quantifiable differences in baseline cTnI concentrations between different rat strains, sexes, and surgical alterations, highlighting the need for strain-specific control data in preclinical models.
Strain- and sex-specific cTnI baselines are required in rat models; leaves open translation to clinical troponin use.
Cardiac troponins (cTnT and I) are reliable biomarkers for detecting myocardial injury. New ultrasensitive assays have been able to detect baseline serum cTn levels even in healthy patients. Recently, an ultrasensitive cTnI immunoassay (Erenna IA, Singulex, CA) has detected increases from baseline cTnI levels due to drug-induced myocardial injury in rats, dogs and monkeys and baseline cTnI ranges in Sprague-Dawley (SD) rats. The present study was initiated to use the Erenna cTnI assay to further document baseline cTnI levels in normal control animals from multiple strains, including spontaneous hypertensive (SHR), SD, Wistar, Wistar-Kyoto, Fisher, SHR ovariectomized and SHR castrated rats. Baseline cTnI concentrations were detected in all 115 rats tested. Male rats had higher mean cTnI concentrations than females of the same strain.(SHR 26 vs 10 pg/ml) SHR male rats had the highest baseline levels (mean=26 pg/ml and the largest cTnI variability(3.9–70.2 pg/ml). Increased cTnI levels were observed for castrated SHR compared to unaltered male SHR, (51 vs 16 pg/ml) while ovariectomized SHR had lower cTnI concentrations than normal female SHR (mean=4 vs 9 pg/ml).. These results show quantifiable differences in cTnI concentrations between strains, sexes and non-cardiac surgical alterations in control animals. Thus, there is a continuing need to develop an expanded knowledge base of control information in order to realize maximal potential from monitoring small changes in cardiac troponin by new ultrasensitive assays.
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Herman et al. (2011) conducted an observational in Healthy control animals (n=115). Strain, sex, and non-cardiac surgical alterations vs. Other strains, opposite sex, or unaltered rats was evaluated on Baseline cTnI concentrations. Baseline cTnI concentrations showed quantifiable differences between rat strains, sexes, and surgical alterations, with male SHR rats having higher levels (26 pg/ml) than females (10 pg/ml).
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