Key result
Polysaccharides from fermented barley bran extract (50 μg/mL) reduced triglyceride content and reactive oxygen species levels in high-fat HepG2 cells by 21.62% and 30.01%, respectively.
Why the study?
Barley bran has potential bioactivity, and fermentation may promote functional activity, but the effects of polysaccharides from fermented barley bran extract on lipid accumulation and oxidative stress remained to be evaluated.
Does FBBE-PS reduce lipid accumulation and oxidative stress in high-fat HepG2 cells?
Does FBBE-PS reduce lipid accumulation and oxidative stress in high-fat HepG2 cells?
Polysaccharides from fermented barley bran extract alleviate lipid deposition and oxidative stress in an in vitro model of high-fat cells.
FBBE-PS may reduce lipid accumulation and oxidative stress in vitro; hypothesis-generating and requires in vivo validation before any clinical consideration.
Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc. Fermentation has been considered as an effective way to promote the functional activity of food raw materials. In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated. The results showed that the molecular weight decreased and monosaccharide composition of polysaccharides changed significantly after fermentation. In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively. RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc. The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression. It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
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Zhao et al. (2023) studied High-fat HepG2 cells induced by sodium oleate. Polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) was evaluated on Triglyceride (TG) content and reaction oxygen species (ROS) level. Polysaccharides from fermented barley bran extract (50 μg/mL) reduced triglyceride content and reactive oxygen species levels in high-fat HepG2 cells by 21.62% and 30.01%, respectively.
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