January 15, 2004Analytical Chemistry

Binding of Fluorescent Dye to Genomic RNA Inside Intact Human Rhinovirus after Viral Capsid Penetration Investigated by Capillary Electrophoresis

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Population

Human rhinovirus serotypes 2 and 14

Design

Preclinical

Authors

LKLeopold KremserInnsbruck Medical University VOVadim M. OkunUniversity of Vienna ANAndreas NicodemouUnited States Department of the Treasury

Discussion

Member takes

Implication

Enables fluorescent labeling of intact virions for single-molecule virology; leaves open in vivo translation and clinical utility.

Structured PICO

Population

Human rhinovirus serotypes 2 and 14

Intervention

Incubation with RiboGreen fluorescent dye

Outcome

Dye penetration and binding to viral RNA, electrophoretic mobility, and conservation of native conformationsurrogate

RiboGreen can penetrate intact human rhinovirus capsids to label viral RNA in vitro, allowing for the creation of fluorescent viral particles with unmodified surfaces for single-molecule studies.

Cite This Study

Kremser et al. (2004) studied this question.

synapsesocial.com/papers/6a881bec4c82be800eeb4483 https://doi.org/10.1021/ac034898x

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Binding of Fluorescent Dye to Genomic RNA Inside Intact Human Rhinovirus after Viral Capsid Penetration Investigated by Capillary Electrophoresis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Binding of Fluorescent Dye to Genomic RNA Inside Intact Human Rhinovirus after Viral Capsid Penetration Investigated by Capillary Electrophoresis

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider