What question did this study set out to answer?

To evaluate the diagnostic accuracy of an enzyme-linked immunosorbent assay (ELISA) measuring anti-tissue transglutaminase (tTG) antibodies compared to the standard anti-endomysial antibody (EMA) immunofluorescence test for coeliac disease.

January 1, 2003British Journal of Biomedical Science

Anti-transglutaminase antibodies and the serological diagnosis of coeliac disease

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Authors

LFLinda FeigheryUCB Pharma (United Kingdom)CCCarol Collins CFC. FeigheryTrinity College Dublin

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Implication

Comparative study reveals anti-transglutaminase ELISA produces more non-specific results than endomysial antibody testing in autoimmune cohorts, indicating lower diagnostic specificity.

Key Points

To evaluate the diagnostic accuracy of an enzyme-linked immunosorbent assay (ELISA) measuring anti-tissue transglutaminase (tTG) antibodies compared to the standard anti-endomysial antibody (EMA) immunofluorescence test for coeliac disease.
Measured serum IgA, IgG, and IgM antibodies to tTG using an ELISA and compared the results to standard immunofluorescence testing for EMA.
Evaluated serum specimens from controls (n=146), EMA-positive individuals (n=29), Graves' disease patients (n=94), antimitochondrial antibody-positive individuals (n=53), rheumatoid arthritis patients (n=53), and systemic lupus erythematosus patients (n=46).
Anti-tTG antibodies were detected in 83% of EMA-positive sera (n=29) and 3% of controls (n=146), whereas EMA tests were positive in 0% of controls.
Non-specific anti-tTG reactivity occurred in 22% of systemic lupus erythematosus, 12% of antimitochondrial antibody-positive, 11% of rheumatoid arthritis, and 9% of Graves' disease samples, compared to EMA positivity rates of 0%, 2%, 2%, and 1%, respectively.
IgA anti-tTG was markedly more reliable than IgG or IgM assays, but anti-tTG ELISA overall demonstrated a higher false-positive rate and lower diagnostic performance than standard EMA testing.

Cite This Study

Feighery et al. (2003) studied this question.

synapsesocial.com/papers/6a88290b86ac178e8f608b66 https://doi.org/10.1080/09674845.2003.11783671

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Implication

Comparative study reveals anti-transglutaminase ELISA produces more non-specific results than endomysial antibody testing in autoimmune cohorts, indicating lower diagnostic specificity.

Anti-transglutaminase antibodies and the serological diagnosis of coeliac disease

Discussion

Member takes

Implication

Key Points

Cite This Study

Anti-transglutaminase antibodies and the serological diagnosis of coeliac disease

Discussion

Member takes

Implication

Key Points

Cite This Study

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