Smooth muscle and cytoplasmic actin isoforms are randomly distributed and co-exist within individual thin filaments of smooth muscle cells, rather than being segregated into separate contractile and cytoskeletal populations.
Challenges segregated actin models in smooth muscle; leaves open functional consequences for contractility and requires human validation.
Smooth muscle cells contain both smooth muscle-specific and cytoplasmic actin isoforms. We tested the hypothesis that these two classes of isoactins are segregated into separate "contractile" vs. "cytoskeletal" populations of thin filaments. Isolated thin filaments from adult swine stomach were double-labeled with isoactin-specific peptide antibodies with the use of two different size protein A-gold markers to localize the isoactins on individual filaments. The large and small gold beads bound to individual filaments were counted on electron micrographs, and the results were analyzed statistically. Both classes of actin isoforms were present in every filament, and the relative proportions of smooth muscle vs. cytoplasmic actin isoforms in each filament showed no significant deviation from a single random population. Furthermore, the distribution of the isoactins along each filament was also random; no significant clustering of isoforms was observed. Controls containing added skeletal muscle F-actin showed that the techniques used could have detected nonrandom isoform distributions if they had been present.
No takes yet. Share an insight, caveat, or question.
Drew et al. (1991) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: