Key result
MyHC-2b transcripts and the corresponding protein isoform are expressed in human laryngeal muscles and co-expressed with major fast and slow MyHC isoforms.
This study provides the first evidence of MyHC-2b transcript and protein expression in human laryngeal muscles.
Does not yet alter laryngeal disorder management; leaves open functional significance of MyHC-2b co-expression.
Three fast myosin heavy chain (MyHC) isoforms, i.e. MyHC-2a, -2x and -2b, are expressed in skeletal muscles of smaller mammals. In contrast, only MyHC-2a and -2x have been revealed in humans so far. The expression of MyHC isoforms is known to be wider in the functionally more specialized laryngeal muscles. Though mRNA transcripts of the MyHC-2b gene were found to be expressed in certain human skeletal and laryngeal muscles, the corresponding isoform has not been demonstrated in these muscles. To our knowledge, we are the first to demonstrate not only the expression of MyHC-2b transcripts using an in situ hybridization technique but also the corresponding protein, i.e. the MyHC-2b isoform, in some human laryngeal muscles by immunohistochemistry but not by polyacrylamide gel electrophoresis. Using a set of antibodies specific to MyHC isoforms, we demonstrated that MyHC-2b was always co-expressed with the major MyHC isoforms, not only with the fast ones (MyHC-2a and -2x) but with the slow isoform (MyHC-1) as well.
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Smerdu et al. (2013) studied this question. MyHC-2b expression was evaluated on Expression of MyHC-2b transcripts and protein. MyHC-2b transcripts and the corresponding protein isoform are expressed in human laryngeal muscles and co-expressed with major fast and slow MyHC isoforms.
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