Mechanism of Ion Permeation in Skeletal Muscle Chloride Channels
Population
Recombinant human skeletal muscle Cl- channel (hClC-1) expressed in Xenopus oocytes and a mammalian cell line
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Does not alter clinical practice; extends molecular models of hClC-1 but leaves therapeutic targeting open pending in vivo validation.
Structured PICO
P
Population
Recombinant human skeletal muscle Cl- channel (hClC-1) expressed in Xenopus oocytes and a mammalian cell line
I
Intervention
Extracellular or intracellular I- and related anions (Br-, SCN-, NO3-, CH3SO3-)
O
Outcome
Ion permeation and gating properties (block by anions, gating alterations)surrogate
The study identifies two functional ion binding sites within the hClC-1 pore and demonstrates that ion binding is modulated by the gating state of the channel.
Cite This Study
Fahlke et al. (1997) studied this question.