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Source/description: A genomic plasmid library was constructed in the plasmid vector pCR-ScriptTM (Stratagene, La Jolla, CA) from size-selected (300–800 bp) rainbow trout genomic DNA digested with PvuII and HaeIII. The library was screened with a (TG)9 probe. Positive clones were sequenced using an automatic DNA sequencer (A.L.F. express DNA Sequencer, Pharmacia, Uppsala, Sweden). The clone Omy0004DIAS was shown to contain a (GT)17 repeat (GenBank accession number AF090389), while clone Omy0008DIAS contained a (GT)11 repeat (GenBank accession number AF090390). PCR primers were designed on the basis of the flanking sequences. The sizes of the PCR fragments were analysed on the A.L.F. express DNA Sequencer.
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Holm et al. (1999) studied this question.
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