Why the study?
Does actinomycin D or chloramphenicol inhibit 19S antibody formation in rabbit spleen cells in vitro?
Does actinomycin D or chloramphenicol inhibit 19S antibody formation in rabbit spleen cells in vitro?
The study demonstrates that 19S antibody formation is dependent on DNA-dependent RNA synthesis, which can be inhibited by actinomycin D or chloramphenicol.
Answers are grounded in this paper and cite their sources.
No clinical implications for immunomodulation; hypothesis-generating for RNA synthesis dependence in 19S antibody responses.
The formation of 19S antibody to poliovirus by rabbit spleen cells in vitro was interrupted by adding actinomycin D (1 to 10 micrograms per milliliter) for 30 minutes or longer. Shorter treatment (5 to 15 minutes) caused partial suppression. Antibody formation was slowly renewed upon removal of the drug. These results suggest that 19S antibody formation is contingent upon DNA-dependent RNA synthesis and that the genetic messenger has an average lifetime of one-half day or less. Treatment with chloramphenicol (20 to 50 micrograms per milliliter) for 3 days caused the cessation of 19S antibody formation.
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Sven‐Erik Svehag (1964) studied this question.
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