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August 21, 2026Journal of the American College of Cardiology

Ethanol Infusion in the Vein of Marshall Leads to Parasympathetic Denervation of the Human Left Atrium

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Why the study?

Intrinsic cardiac nerves (ICN) cluster around the left atrial epicardium and are implicated in the genesis of AF, but whether ethanol infusion in the VOM can ablate ICN was unknown.

Does ethanol infusion in the vein of Marshall eliminate parasympathetic responses in patients undergoing atrial fibrillation ablation?

Population

Patients undergoing catheter AF ablation (n=8 SynchHFS, n=72 BurstHFS)

Comparison

Before vs after VOM ethanol infusion

Design

Prospective interventional study

Key result

Retrograde ethanol infusion in the vein of Marshall abolished local parasympathetic responses in 100% of patients undergoing atrial fibrillation ablation.

Authors

JBJosé L. Báez-EscuderoTKTakehiko KeidaADAmish S. Dave

Discussion

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Member takes

Key expert perspectives

Captured external expert commentary on this paper, strongest first. Original sources are linked where available.

MVMiguel ValderrábanoChief of cardiac electrophysiology, Houston Methodist

“This is a therapy that targets cardiac nerves previously shown to be involved in atrial fibrillation. Radiofrequency ablation carries risks of collateral damage to other structures and there are also risks associated with surgical approaches. We show that chemical ablation with alcohol can achieve elimination of abnormal nerve activity, introducing a catheter through a neck vein and doing all the work through it.”

Houston Methodist DeBakey Heart & Vascular CenterNews Coverage
KSKalyanam ShivkumarDirector, UCLA Cardiac Arrhythmia Center

“the VOM is absent or too small to be cannulated in some patients, potentially increasing procedure time”

UCLA Cardiac Arrhythmia CenterEditorial

Overview

May attenuate ICN activity during AF ablation; hypothesis-generating and requires randomized trials before practice change.

Key Points

  • Determine whether retrograde ethanol infusion into the vein of Marshall can ablate intrinsic cardiac nerves implicated in atrial fibrillation.
  • Evaluated 80 patients undergoing catheter atrial fibrillation (AF) ablation, delivering up to four 1 mL infusions of 98% ethanol via an angioplasty balloon in the vein of Marshall (VOM).
  • Applied high-frequency stimulation (HFS) via multipolar catheter in the VOM as P-wave-synchronized (SynchHFS, n=8) or burst stimulation (BurstHFS, n=72; 32 de novo [Group 1] and 40 repeat [Group 2]).
  • Measured atrioventricular nodal conduction slowing (asystole >2s or R-R prolongation >50%) and AF inducibility before and after ethanol delivery.
  • Baseline BurstHFS induced parasympathetic responses in 100% of de novo patients (32/32) and 75% of repeat ablation patients (30/40), while SynchHFS triggered AF in 100% (8/8).
  • Retrograde VOM ethanol infusion abolished parasympathetic responses in 100% of patients across both de novo and repeat groups.
  • Zero acute complications occurred during or after VOM ethanol infusion.

Structured PICO

Does ethanol infusion in the vein of Marshall eliminate parasympathetic responses in patients undergoing atrial fibrillation ablation?

P
Population
80 patients undergoing catheter atrial fibrillation ablation.
I
Intervention
Adjunctive ethanol injection in the vein of Marshall (VOM) (up to four 1mL infusions of 98% ethanol delivered via an angioplasty balloon).
C
Comparator
Baseline (before VOM ethanol infusion).
O
Outcome
Parasympathetic responses (atrioventricular nodal conduction slowing defined as asystole >2s or R-R interval prolongation >50%) and AF inducibility.surrogate

Retrograde ethanol infusion in the vein of Marshall reliably eliminates local intrinsic cardiac nerve responses, offering a targeted vascular approach for parasympathetic denervation during AF ablation.

Cite This Study

Báez-Escudero et al. (2014) studied Atrial fibrillation (n=80). Ethanol infusion in the vein of Marshall was evaluated on Parasympathetic responses (AV nodal conduction slowing). Retrograde ethanol infusion in the vein of Marshall abolished local parasympathetic responses in 100% of patients undergoing atrial fibrillation ablation.

synapsesocial.com/papers/6a88c0c1f0c2079f0c0124d8https://doi.org/10.1016/j.jacc.2014.01.032
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