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PRINCIPLES OF THE RECOMBINANT DNA APPROACH ••..••..• •.•.••.• •••..•• 404 GENETIC MANIPULATION 406 Choice of Marker Protein 406 Engineering within Coding Sequences 408 Expression Systems 409 INSERTION OF PROTEINS INTO AND ACROSS THE MEMBRANE OF THE ER .. ... . 41 1 Cotranslational Signals for Protein Topogenesis in the ER ... . ... ....... 411 The Signal Peptide 415 Permanent ER Insertion Sequences 418 The Stop Transfer Signal 420
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Henrik Garoff (1985) studied this question.
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