What question did this study set out to answer?

To determine the biological requirements for efficient lentiviral vector-mediated gene transfer into liver cells in living animal models.

March 1, 2001Molecular TherapyOpen Access

Transduction of Liver Cells by Lentiviral Vectors: Analysis in Living Animals by Fluorescence Imaging

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Authors

APAlexander PfeiferVascular Medicine TKTorsten KeßlerKlinik für Schlafmedizin MYMeng YangChina-Japan Friendship Hospital

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Implication

Animal study demonstrates efficient lentiviral gene transfer into murine liver without requiring cell division, indicating strong promise for liver-directed gene therapies.

Key Points

To determine the biological requirements for efficient lentiviral vector-mediated gene transfer into liver cells in living animal models.
Administered advanced-generation lentiviral vectors encoding fluorescent reporter genes, including green fluorescent protein, into mice.
Visualized and analyzed vector-mediated reporter gene expression within liver tissues of living animals using a noninvasive fluorescence imaging system.
Assessed the requirement of target cell-cycle progression and cell division for successful in vivo hepatic transduction.
Latest-generation lentiviral vectors efficiently transduce murine liver cells in vivo.
Lentiviral gene transfer occurs effectively without requiring liver cell division or cell-cycle activation.

Cite This Study

Pfeifer et al. (2001) studied this question.

synapsesocial.com/papers/6a89004af1df03d55b28b316 https://doi.org/10.1006/mthe.2001.0276

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Implication

Animal study demonstrates efficient lentiviral gene transfer into murine liver without requiring cell division, indicating strong promise for liver-directed gene therapies.

Transduction of Liver Cells by Lentiviral Vectors: Analysis in Living Animals by Fluorescence Imaging

Discussion

Member takes

Implication

Key Points

Cite This Study

Transduction of Liver Cells by Lentiviral Vectors: Analysis in Living Animals by Fluorescence Imaging

Discussion

Member takes

Implication

Key Points

Cite This Study

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