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June 4, 2013Chinese Science BulletinOpen Access

Progress of AFM single-cell and single-molecule morphology imaging

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Authors

MLMi LiGuangxi UniversityLLLianqing LiuJilin UniversityNXNing XiXidian University

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Implication

Narrative review highlights advances in atomic force microscopy for living cells and native membrane proteins, suggesting expanded roles in high-resolution nanoscale biology.

Key Points

  • Summarize recent methodological advances, sample preparation protocols, and remaining challenges in atomic force microscopy (AFM) for high-resolution imaging of living cells and native membrane proteins.
  • Reviewed sample preparation protocols required for stabilizing soft biological specimens during single-cell and single-molecule AFM imaging in natural fluid environments.
  • Synthesized technical developments and imaging workflows spanning from early 1990s AFM studies to modern biological applications.
  • Atomic force microscopy enables label-free, high-resolution topographical imaging of living cells and native membrane proteins under physiological fluid conditions.
  • Progress in sample preparation and tip-sample interactions has extended AFM resolution, though maintaining non-destructive, high-resolution imaging across soft cellular surfaces remains a primary challenge.

Cite This Study

Li et al. (2013) studied this question.

synapsesocial.com/papers/6a89075fab71709247a2acfchttps://doi.org/10.1007/s11434-013-5906-z
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