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April 1, 1999Journal of Biological ChemistryOpen Access

Modulation of the RNA Binding and Protein Processing Activities of Poliovirus Polypeptide 3CD by the Viral RNA Polymerase Domain

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Population

Recombinant 3C and 3CD polypeptides (poliovirus)

Comparison

Mutations in the 3D domain of 3CD vs Wild type 3C and 3CD

Design

Preclinical

Authors

TPTodd B. ParsleyBiotechnology InstituteCCChristopher T. CornellScripps Research InstituteBSBert L. SemlerUniversity of California, Irvine

Discussion

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Overview

3D domain critically modulates 3CD protease efficiency and RNA binding; extends viral maturation models but leaves open therapeutic targeting.

Structured PICO

P
Population
Recombinant 3C and 3CD polypeptides (poliovirus)
I
Intervention
Mutations in the 3D domain of 3CD
C
Comparator
Wild type 3C and 3CD
O
Outcome
Proteinase substrate recognition and RNA binding properties (cleavage efficiency of viral polyprotein precursors)surrogate

The RNA polymerase domain (3D) of poliovirus polypeptide 3CD plays a critical role in modulating its proteinase activity and RNA binding properties.

Cite This Study

Parsley et al. (1999) studied this question.

synapsesocial.com/papers/6a891d18d5f4e697bb132441https://doi.org/10.1074/jbc.274.18.12867
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Poliovirus Protein 3CD Is the Active Protease for Processing of the Precursor Protein P1 in vitro1988 · 186 citations
  2. 2Genetic dissection of interaction between poliovirus 3D polymerase and viral protein 3AB1997 · 114 citations
  3. 3Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.1982 · 250 citations
  4. 4Structural domains of the poliovirus polyprotein are major determinants for proteolytic cleavage at Gln-Gly pairs.1988 · 92 citations
  5. 5Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis1994 · 99 citations