What question did this study set out to answer?

To determine the relative contributions of N-linked and O-linked oligosaccharides to the increased molecular weight of free gonadotropin alpha subunit compared to its dimerized form.

October 1, 1987Journal of Biological ChemistryOpen Access

Gonadotropin alpha subunit. Differential processing of free and combined forms in human trophoblast and transfected mouse cells.

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To determine the relative contributions of N-linked and O-linked oligosaccharides to the increased molecular weight of free gonadotropin alpha subunit compared to its dimerized form.
Analyzed free alpha subunits and combined human chorionic gonadotropin alpha secreted by human first-trimester placental explants, cultured cytotrophoblasts, and transfected C-127 mouse mammary tumor cells.
Evaluated subunit glycosylation structures using tryptic peptide mapping, the processing inhibitor swainsonine, and endoglycosidase H enzymatic digestion.
Tryptic mapping showed that O-glycosylation at threonine 39 was not a major modification in either placental or transfected alpha subunits.
Inhibition with swainsonine eliminated the molecular weight discrepancy between free and combined alpha forms in both cell systems, confirming differential N-linked oligosaccharide processing.
Free alpha subunits exhibited resistance to endoglycosidase H cleavage, whereas combined dimer forms remained partially sensitive to the enzyme.

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Implication

Laboratory study demonstrates differential N-linked oligosaccharide processing between free and dimerized gonadotropin alpha in trophoblasts, indicating subunit pairing alters glycan maturation.

Cite This Study

Corless et al. (1987) studied this question.

synapsesocial.com/papers/6a892ef71a276c874728d001 https://doi.org/10.1016/s0021-9258(18)47923-9

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Implication

Laboratory study demonstrates differential N-linked oligosaccharide processing between free and dimerized gonadotropin alpha in trophoblasts, indicating subunit pairing alters glycan maturation.

Gonadotropin alpha subunit. Differential processing of free and combined forms in human trophoblast and transfected mouse cells.

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Discussion

Implication

Cite This Study

Gonadotropin alpha subunit. Differential processing of free and combined forms in human trophoblast and transfected mouse cells.

Key Points

Discussion

Implication

Cite This Study

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