Population
Porcine atrial membranes enriched in muscarinic acetylcholine (mACh) receptors
Comparison
Carbachol stimulation in the presence of GDP and… vs Control binding without agonist or with GDP alone
Design
Preclinical
Authors
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May amplify vagal signaling in cardiac membranes; leaves open translation to human physiology or intact myocytes.
The study demonstrates that agonist-stimulated binding of [35S]GTP[S] is a sensitive assay for measuring receptor-regulated G-protein activation in native cardiac membranes.
Hilf et al. (1989) studied this question.