March 11, 1986Biochemistry

Solvent accessibility of the adenosine 5'-triphosphate catalytic site of sarcoplasmic reticulum calcium ATPase

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Population

Rabbit skeletal sarcoplasmic reticulum CaATPase

Comparison

Labeling with fluoresceinyl isothiocyanate and… vs Free FITC

Design

Preclinical

Authors

SHStefan HighsmithUniversity of the Pacific

Discussion

Member takes

Implication

Should not yet inform clinical calcium handling decisions; leaves open conformational dynamics of human CaATPase for further study.

Structured PICO

Population

Rabbit skeletal sarcoplasmic reticulum CaATPase

Intervention

Labeling with fluoresceinyl isothiocyanate (FITC) and exposure to various ligands (Ca2+, vanadate, phosphate) across pH range 5-8

Comparator

Free FITC

Outcome

Solvent accessibility determined by I- quenching of fluorescence (collisional quenching rate constants)surrogate

The study demonstrates that ligand binding induces conformational changes in the ATP catalytic site of sarcoplasmic reticulum CaATPase, altering its solvent accessibility.

Cite This Study

Stefan Highsmith (1986) studied this question.

synapsesocial.com/papers/6a894487176d2ac34cfb901e https://doi.org/10.1021/bi00353a015

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Solvent accessibility of the adenosine 5'-triphosphate catalytic site of sarcoplasmic reticulum calcium ATPase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Solvent accessibility of the adenosine 5'-triphosphate catalytic site of sarcoplasmic reticulum calcium ATPase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider