Why the study?
Does alphaxalone block acetylcholine-induced ionic currents in cultured myoballs?
Does alphaxalone block acetylcholine-induced ionic currents in cultured myoballs?
Alphaxalone produces an 'all or none' block of active (open) acetylcholine-induced ionic channels.
Does not alter clinical alphaxalone use; leaves open whether open-channel block contributes to in vivo anesthesia.
The effects of the steroid anaesthetic alphaxalone on acetylcholine (ACh)-induced ionic channels were studied in voltage clamped 'myoballs' in culture. Alphaxalone produced a reversible blockade of the ACh-evoked inward current, ED50 = 6.0 microM. The ACh reversal potential (-5.0 mV), the single channel conductance (13.5 pS) and mean open time (3.6 ms) were unchanged by the drug. Thus, alphaxalone produced an 'all or none' block of the ionic channel. In double pulse conditioning experiments, alphaxalone produced an additional inhibition with a time constant of recovery (550 ms) much longer than the time constant of recovery of the normal desensitization (250 ms). It was concluded that alphaxalone blocks active (open) ionic channels.
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Gillo et al. (1984) studied this question.
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