Melting from Both Ends of an A-Band in a Myofibril, Observation with a Phase-Contrast Microscope1

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Population

rabbit skeletal myofibril

Design

Preclinical

Authors

Discussion

Bidirectional A-band melting in rabbit myofibrils extends in vitro sarcomere models; leaves open relevance to in vivo physiology or disease.

Population

rabbit skeletal myofibril

Intervention

high concentrations of KCl and PPi

Outcome

melting of an A-band (bundle of thick myosin filaments)

Demonstrates that A-band melting in rabbit skeletal myofibrils occurs from both ends under high KCl and PPi concentrations.

Shin’ichi Ishiwata (1981) studied this question.

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Bidirectional A-band melting in rabbit myofibrils extends in vitro sarcomere models; leaves open relevance to in vivo physiology or disease.