Key result
Activated rabbit muscle fibers exhibit ~100-fold higher cleavage rates vs relaxed fibers, linking conformation to force.
Population
Rabbit skeletal glycerinated muscle fibers
Comparison
Activation in the presence of an… vs Rigor or relaxed muscle fibers
Design
Preclinical
Authors
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Does not support clinical translation to human muscle disorders; leaves open further mechanistic studies of myosin hinge dynamics in contraction.
Effect estimate: 100 times larger
There is a close coupling between the conformational transition within the myosin subfragment-2 hinge domain and contractile force during cross-bridge cycling in skeletal muscle.
Ueno et al. (1986) studied this question. ATP-regenerating system (activation) vs. Rigor or relaxed muscle fibers was evaluated on Cleavage rate constant within the subfragment-2 hinge domain (100 times larger). Activated rabbit muscle fibers had a cleavage rate constant about 100 times larger than rigor or relaxed fibers, suggesting a close coupling between conformational transition and contractile force.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: