Comparative Effects of GABA, 5-Aminolevulinic Acid, and Bacillus-Based Treatments on IBA-Pretreated Tea Chrysanthemum Cuttings Under Plateau Cultivation Conditions
Authors
Discussion
Loading...
Member takes
Implication
Controlled trial demonstrates that GABA treatment enhances photosynthesis, antioxidant defenses, and growth in tea chrysanthemum cuttings, suggesting strong potential for harsh cultivation climates.
Key Points
- To compare the regulatory effects of GABA, 5-aminolevulinic acid, and Bacillus-based biostimulants on the rooting, physiology, and growth of IBA-pretreated tea chrysanthemum cuttings under plateau stress.
- Conducted a 60-day plug-tray experiment with three biological replicates using tea chrysanthemum cuttings pretreated with 500 mg·L−1 indole-3-butyric acid (IBA).
- Applied four separate treatments: 5-aminolevulinic acid hydrochloride (5-ALA), γ-aminobutyric acid (GABA), Bacillus amyloliquefaciens, and Bacillus velezensis alongside an IBA control.
- Assessed root morphology, seedling vigor, leaf gas exchange, oxidative stress biomarkers (MDA, H2O2, APX), and rhizosphere nutrient parameters.
- GABA treatment improved net photosynthetic rate by 316.34%, stomatal conductance by 92.31%, transpiration rate by 168.00%, plant height by 60.85%, stem diameter by 46.08%, and seedling vigor index by 21.74%.
- GABA enhanced ascorbate peroxidase (APX) activity and soluble sugar levels while decreasing malondialdehyde (MDA) and hydrogen peroxide (H2O2) accumulation; 5-ALA uniquely enhanced rooting rate and seedling survival.
- Comprehensive D-value multi-trait evaluation ranked overall regulatory efficacy as GABA > B. amyloliquefaciens > 5-ALA > B. velezensis > control.
Cite This Study
Zhao et al. (2026) studied this question.