Independent Multi-Cohort RNA-Seq Analysis Does Not Support Recurrent DNAJB1::PRKACA Fusion in Hepatoblastoma or Biliary Atresia. Comment on Fleifil et al. DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways. Cancers 2025, 17, 83

Q: What does this research mean for the field?

Independent multi-cohort RNA-seq analysis demonstrates that the DNAJB1::PRKACA fusion is not recurrently present in hepatoblastoma or biliary atresia, refuting recent claims of its expression in these pediatric liver conditions. Novelty: ClaimNovelty.CONTRADICTORY. Consensus alignment: ConsensusAlignment.CHALLENGES_CONSENSUS.

Fahreddin Palaz; Xuanxuan Yu; Julia Bruner; Michael Feely; Guoshuai Cai; Sergio Duarte; Ali Zarrinpar

doi:10.3390/cancers18162681

August 19, 2026CancersOpen Access

Independent Multi-Cohort RNA-Seq Analysis Does Not Support Recurrent DNAJB1::PRKACA Fusion in Hepatoblastoma or Biliary Atresia. Comment on Fleifil et al. DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways. Cancers 2025, 17, 83

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Key Points

To independently evaluate whether the oncogenic DNAJB1::PRKACA fusion gene recurrently occurs in hepatoblastoma and biliary atresia patient cohorts.
Performed multi-cohort RNA sequencing (RNA-Seq) transcriptomic analysis across independent datasets of pediatric hepatoblastoma and biliary atresia cases.
Assessed fusion transcript reads, structural breakpoints, and expression levels relative to established liver cancer molecular profiles.
No recurrent DNAJB1::PRKACA fusion transcripts were detected across independent hepatoblastoma or biliary atresia cohorts.
The findings refute recent claims of widespread DNAJB1::PRKACA involvement in non-fibrolamellar pediatric liver pathologies.

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Implication

Multi-cohort transcriptomic analysis reveals an absence of recurrent DNAJB1-PRKACA fusions in hepatoblastoma and biliary atresia, indicating limited diagnostic utility outside fibrolamellar tumors.

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Palaz et al. (2026) studied this question.

synapsesocial.com/papers/6a895ed9ca7ade938187d0fd https://doi.org/10.3390/cancers18162681

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Independent Multi-Cohort RNA-Seq Analysis Does Not Support Recurrent DNAJB1::PRKACA Fusion in Hepatoblastoma or Biliary Atresia. Comment on Fleifil et al. DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways. Cancers 2025, 17, 83

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Cite This Study

Independent Multi-Cohort RNA-Seq Analysis Does Not Support Recurrent DNAJB1::PRKACA Fusion in Hepatoblastoma or Biliary Atresia. Comment on Fleifil et al. DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways. Cancers 2025, 17, 83

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Cite This Study

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