What question did this study set out to answer?

To isolate, phenotypically characterize, and determine the recruitment mechanisms of a distinct population of circulating human non-adherent endothelial forming cells.

November 7, 2012PLoS ONEOpen Access

Characterization of a Distinct Population of Circulating Human Non-Adherent Endothelial Forming Cells and Their Recruitment via Intercellular Adhesion Molecule-3

View Full Paper

Ask AI

Bookmark

Authors

SASarah ApplebyUniversity of Otago MCMichaelia P. CockshellThomas Jefferson University JPJyotsna B. PippalMedical University of Graz

Discussion

Member takes

Implication

Experimental study reveals a distinct population of human non-adherent endothelial forming cells recruited via ICAM-3, suggesting a novel target to regulate vasculogenesis.

Key Points

To isolate, phenotypically characterize, and determine the recruitment mechanisms of a distinct population of circulating human non-adherent endothelial forming cells.
Enriched CD133(+) cells from human umbilical cord blood and characterized cell surface markers using flow cytometry, genomics, and proteomics.
Assayed endothelial phenotypes via lectin binding, acetylated-LDL uptake, TNF-stimulated VCAM-1 expression, 3D in vitro tubulogenesis, and in vivo vessel formation.
Evaluated recruitment kinetics including cell rolling and adhesion under shear stress mediated by intercellular adhesion molecule-3 (ICAM-3).
Non-adherent endothelial forming cells (naEFCs) co-expressed stem cell markers (CD133, CD34, CD117, CD90, CD38) and mature endothelial markers (VEGFR2, CD144, CD31) without lymphoid or myeloid markers.
naEFCs promoted tube branching and network formation in 3D matrices in vitro and spontaneously generated lumenized, CD144(+) human blood vessels in vivo.
ICAM-3 was uniquely expressed on naEFCs compared with mature endothelial cells and directly mediated cell rolling and adhesive capture under physiological shear stress.

Cite This Study

Appleby et al. (2012) studied this question.

synapsesocial.com/papers/6a89617dde65451374621ac2 https://doi.org/10.1371/journal.pone.0046996

View Full Paper

Ask AI

Bookmark

Implication

Experimental study reveals a distinct population of human non-adherent endothelial forming cells recruited via ICAM-3, suggesting a novel target to regulate vasculogenesis.

Characterization of a Distinct Population of Circulating Human Non-Adherent Endothelial Forming Cells and Their Recruitment via Intercellular Adhesion Molecule-3

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of a Distinct Population of Circulating Human Non-Adherent Endothelial Forming Cells and Their Recruitment via Intercellular Adhesion Molecule-3

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider