Key result
In cattle recovering from FMDV serotype 'O', IL-1α, IFN-α, and TNF-α genes were up-regulated up to 3 months post-infection, whereas IL-1β was down-regulated.
Observational
Real-time PCR is a reliable technique for detecting persistent FMDV and profiling cytokine expression in recovered cattle.
Cytokine patterns may signal FMDV persistence in cattle; hypothesis-generating for livestock immunity and PCR-based monitoring.
The molecular epidemiological study of foot-and-mouth disease virus (FMDV) has been carried out from different outbreaks in Assam the present study is based on the nucleotide sequencingof circulating FMDV serotype. The samples were subjected to sandwich ELISA, multiplex-PCR and molecular phylogeny to identify the type species. The phylogenetic analysis of virus sequence revealed similarity with theBangladesh isolates in the major branching pattern. The serotype ‘O’has found to be dominant and responsible for most of the recentoutbreaks.Thepersistence of serotype ‘O’ and cytokines expression of IL-1á, IL-1â, IFN-á, TNF-á in blood of recovered animals were done by Real time PCR. The findings indicated that IL-1á, IFN-á and TNF-á genes were up-regulated upto 3 months post infection but IL-1â found to be down regulated with progression of recovery. The present study thus supports that real-time PCR is a powerful technique for reliable detection of persistent FMDV in recovered animals.
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Baro et al. (2018) conducted an observational in Foot-and-mouth disease virus (FMDV). Foot-and-mouth disease virus (FMDV) infection was evaluated on Persistence of serotype 'O' and cytokines expression (IL-1α, IL-1β, IFN-α, TNF-α). In cattle recovering from FMDV serotype 'O', IL-1α, IFN-α, and TNF-α genes were up-regulated up to 3 months post-infection, whereas IL-1β was down-regulated.
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