Key result
The ethyl acetate extract of Tian-hua-fen demonstrated a dose-dependent protective effect on HeLa cells infected with coxsackievirus B3 by inhibiting viral attachment, entry, biosynthesis, and assembly.
The ethyl acetate extract of Tian-hua-fen demonstrates dose-dependent protective effects against coxsackievirus B3 infection in HeLa cells in vitro.
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Does not support clinical use; leaves open further preclinical development of Tian-hua-fen extract against coxsackievirus.
AIM: To investigation the anti-coxsackievirus B(3) (CVB(3m)) effect of the ethyl acetate extract of Tian-hua-fen on HeLa cells infected with CVB(3m). METHODS: HeLa cells were infected with CVB(3m) and the cytopathic effects (CPE) were observed through light microscope and crystal violet staining on 96-well plate and A(600) was detected using spectrophotometer. The protective effect of the extract to HeLa cells and the mechanism of the effect were also evaluated through the change of CPE and value of A(600). RESULTS: The extract had some toxicity to HeLa cells at a higher concentration while had a marked inhibitory effect on cell pathological changes at a lower concentration. Consistent results were got through these two methods. We also investigated the mechanism of its anti-CVB(3m) effect and the results indicated that the extract represented an inhibitory effect through all the processes of CVB(3m) attachment, entry, biosynthesis and assemble in cells. CONCLUSION: The results demonstrate that the ethyl acetate extract of Tian-hua-fen has a significant protective effect on HeLa cells infected with CVB(3m) in a dose-dependent manner and this effect exists through the process of CVB(3m) attachment, entry, biosynthesis and assemble in cells, suggesting that the ethyl acetate extract of Tian-hua-fen can be developed as an anti-virus agent.
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Li et al. (2004) studied Coxsackievirus B3 infection (in vitro). Ethyl acetate extract of Tian-hua-fen was evaluated on Cytopathic effects (CPE) and A(600) value. The ethyl acetate extract of Tian-hua-fen demonstrated a dose-dependent protective effect on HeLa cells infected with coxsackievirus B3 by inhibiting viral attachment, entry, biosynthesis, and assembly.
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