March 20, 1996Human Gene Therapy

Stable MLV-VL30 Dicistronic Retroviral Vectors with a VL30 or MoMLV Sequence Promoting Both Packaging of Genomic RNA and Expression of the 3′ Cistron

View Full Paper

Ask AI

Bookmark

Population

Cells infected with dicistronic retroviral vectors designed to express human placental alkaline phosphatase…

Design

Preclinical

Follow-up

prolonged culture

Authors

CTChristophe TorrentInstitut Gustave Roussy CBClarisse Berlioz‐TorrentCentre National de la Recherche Scientifique JDJean‐Luc DarlixCentre National de la Recherche Scientifique

Discussion

Member takes

Implication

Advances dicistronic vector feasibility in preclinical models; leaves open translation to human gene therapy.

Structured PICO

Population

Cells infected with dicistronic retroviral vectors designed to express human placental alkaline phosphatase (plap) and neomycin phosphotransferase (neo)

Intervention

Dicistronic retroviral vectors containing the VL30 (positions 205–794) and MoMLV (positions 210–1,035) 5′ sequences between two cistrons

Outcome

Synthesis of exogenous proteins, viral titer, and genetic stability of the integrated recombinant provirussurrogate

The use of VL30 and MoMLV 5' leader sequences with IRES and packaging functions enables the creation of stable, high-titer dicistronic retroviral vectors for efficient coexpression of two exogenous genes.

Cite This Study

Torrent et al. (1996) studied this question.

synapsesocial.com/papers/6a897c88abe412682d92e8ef https://doi.org/10.1089/hum.1996.7.5-603

View Full Paper

Ask AI

Bookmark

Stable MLV-VL30 Dicistronic Retroviral Vectors with a VL30 or MoMLV Sequence Promoting Both Packaging of Genomic RNA and Expression of the 3′ Cistron

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Stable MLV-VL30 Dicistronic Retroviral Vectors with a VL30 or MoMLV Sequence Promoting Both Packaging of Genomic RNA and Expression of the 3′ Cistron

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider