Varying the position of a retrovirus packaging sequence results in the encapsidation of both unspliced and spliced RNAs
Population
Retroviral construct derived from Moloney murine leukemia virus capable of expressing the dominant…
Comparison
Moving or deleting the packaging sequence (psi) vs Wild-type position of psi near the 5' end of the…
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Psi positioning and orientation dictate retroviral titers; hypothesis-generating for vector design optimization.
Structured PICO
P
Population
Retroviral construct derived from Moloney murine leukemia virus capable of expressing the dominant selectable neo gene
I
Intervention
Moving or deleting the packaging sequence (psi)
C
Comparator
Wild-type position of psi (in SVX virus) near the 5' end of the RNA
O
Outcome
Titer of infectious virus production and encapsidation of spliced/unspliced RNAssurrogate
Moving the retrovirus packaging sequence to an exonic region results in the encapsidation, reverse transcription, and integration of both spliced and unspliced RNAs.
Cite This Study
Mann et al. (1985) studied this question.