September 1, 1998Annals of the New York Academy of Sciences

High‐Level Coexpression of the Canine Cardiac Calcium Pump and Phospholamban in Sf21 Insect Cells

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Population

Sf21 insect cells expressing canine cardiac calcium pump (SERCA2a) and phospholamban

Comparison

Coexpression of wild-type phospholamban or… vs Recombinant Ca2+ pump alone or with different…

Design

Preclinical

Authors

JAJoseph M. AutryUniversity of Maryland, Baltimore LJLarry R. JonesUniversity of Vermont

Discussion

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Implication

Mechanistic insights into phospholamban Ca2+ regulation from animal data; leaves open translation to human heart failure therapies.

Structured PICO

Population

Sf21 insect cells expressing canine cardiac calcium pump (SERCA2a) and phospholamban

Intervention

Coexpression of wild-type phospholamban or phospholamban with point mutations in the transmembrane region

Comparator

Recombinant Ca2+ pump alone or with different phospholamban variants

Outcome

Ca2+ transport and ATP hydrolysis (SERCA2a activity)surrogate

The phospholamban monomer may be the active species inhibiting the Ca2+ pump in the cardiac sarcoplasmic reticulum membrane, providing insights into cardiac calcium regulation.

Cite This Study

Autry et al. (1998) studied this question.

synapsesocial.com/papers/6a8984f307adfc24ebcb069a https://doi.org/10.1111/j.1749-6632.1998.tb08259.x

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High‐Level Coexpression of the Canine Cardiac Calcium Pump and Phospholamban in Sf21 Insect Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

High‐Level Coexpression of the Canine Cardiac Calcium Pump and Phospholamban in Sf21 Insect Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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