What question did this study set out to answer?

The study aims to determine the functional role of pentameric versus monomeric phospholamban in cardiac regulation in vivo.

December 1, 1998Journal of Biological ChemistryOpen Access

Pentameric Assembly of Phospholamban Facilitates Inhibition of Cardiac Function in Vivo

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Population

Phospholamban knockout mice

Comparison

Reintroduction of wild-type phospholamban vs Reintroduction of monomeric mutant phospholamban

Design

Preclinical

Authors

GCGuoxiang ChuShandong University of Science and Technology LLLi LiBGI Group (China)YSYoji SatoNational Institute of Health Sciences

Discussion

Member takes

Implication

Animal data implicate pentameric phospholamban in optimal relaxation; leaves open therapeutic targeting in human heart failure.

Key Points

The study aims to determine the functional role of pentameric versus monomeric phospholamban in cardiac regulation in vivo.
Transgenic lines of phospholamban knockout mice were created to express either wild-type or mutant phospholamban.
Sarcoplasmic reticulum Ca2+ transport assays and isolated cardiomyocyte experiments measured contractility and relaxation parameters.
In vivo hemodynamic assessments compared cardiac function between mutant and wild-type phospholamban hearts.
Wild-type phospholamban decreased Ca2+ affinity of SERCA2 similar to the mutant, but greater depression of cardiac relaxation was observed with wild-type (p < 0.05).
Langendorff perfusion studies showed mutant phospholamban less effective in reducing enhanced relaxation in knockout hearts compared to wild-type.
In vivo assessments indicated a significant greater depression of cardiac function with wild-type vs. mutant phospholamban (p < 0.01).

Structured PICO

Population

Phospholamban knockout mice

Intervention

Reintroduction of wild-type (pentameric) phospholamban

Comparator

Reintroduction of monomeric mutant (C41F) phospholamban

Outcome

Cardiac function and relaxation parameters (time constants of relaxation, Ca2+ transient decline, and in vivo hemodynamics)surrogate

Pentameric assembly of phospholamban is necessary for optimal regulation of myocardial contractility in vivo.

Cite This Study

Chu et al. (1998) studied this question.

synapsesocial.com/papers/6a75a3f6ef3d015f2ddd7f70 https://doi.org/10.1074/jbc.273.50.33674

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Pentameric Assembly of Phospholamban Facilitates Inhibition of Cardiac Function in Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Pentameric Assembly of Phospholamban Facilitates Inhibition of Cardiac Function in Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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