Exosome-like vesicles released from ob/ob mouse adipose tissue enhance cell survival of cells with radiation-induced genomic instability

Figures

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  Fig. 1. Treatment of ELVs released from mouse adipose on mouse fibroblast cell line, m5S. (A) A schematic view of the experimental protocol. (B) Detection of CD9 and CD63 proteins in ELVs from control and ob/ob mouse adipose tissues by Western blot. (C) Representative images of PKH67-labeled ELV uptake in m5S cells. Nuclei were counterstained with DAPI. Scale bar: 10 μm.

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  Fig. 2. Effect of ELVs from mouse adipose on colony formation ability in m5S cells. Number of colonies in m5S cells after 0 or 3 Gy irradiation with and without 2 μg/ml ELV treatment from control and ob/ob mouse adipose tissues (ELV-, ELV+) (A), and with and without 4 μg/ml ELV treatment from control and ob/ob mouse adipose tissues (ELV-, ELV+) (B). All data are expressed as Mean ± SD (n = 3). *The differences were significant based on Student’s t-test (P < 0.01).

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  Fig. 3. Effect of ELVs from mouse adipose on radiation-induced genomic instability measured by MN frequency in m5S cells. (A) Representative images of MN (arrowhead) in a m5S cell stained with DAPI. Scale bar: 10 μm (B) MN frequency in m5S cells after 0 or 3 Gy irradiation, with and without 2 or 4 μg/ml ELV treatment from control and ob/ob mouse adipose tissues (ELV-, ELV+). All data are expressed as Mean ± SD (n = 3). *The differences were significant based on Welch’s t-test (P < 0.01).

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  Fig. 4. Effect of ELVs from mouse adipose on colony size and a schematic proposed model of the effect of adipose ELVs on radiation sensitivity. (A) Size of colonies after 0 or 3 Gy irradiation with and without ELV treatment (2 or 4 μg/ml) from control and ob/ob mouse adipose tissues (ELV-, ELV+). (B) Schematic proposed model of the effect of ELVs from obese mouse adipose tissues on cells with genomic instability.