What question did this study set out to answer?

To determine whether the transforming function of Rous sarcoma virus can trigger cell cycle re-entry and oncogenic transformation in quiescent fibroblasts deprived of serum.

May 1, 1975Journal of General Virology

Transformation by a Temperature Sensitive Mutant of Rous Sarcoma Virus in the Absence of Serum

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Authors

JBJ.G. BellInstitut Agronomique et Vétérinaire Hassan II JWJ A WykeThe Honourable Society of Lincoln's Inn IMIan MacphersonThe Wistar Institute

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Implication

Experimental study reveals cell transformation and cycle entry in serum-starved fibroblasts after activating Rous sarcoma virus, indicating viral oncogenes drive proliferation without serum.

Key Points

To determine whether the transforming function of Rous sarcoma virus can trigger cell cycle re-entry and oncogenic transformation in quiescent fibroblasts deprived of serum.
Arrested chicken embryo fibroblasts infected with the temperature-sensitive Rous sarcoma virus mutant tsLA24PR-A between mitosis and S phase by incubation in serum-free medium at 41°C for 2 days.
Shifted arrested cells to the permissive temperature of 35°C to induce transformation, then monitored cell morphology, [2-³H]-deoxy-glucose uptake, [³H]-thymidine incorporation, and viral release.
Lowering culture temperature to 35°C induced transformed cell morphology, elevated [2-³H]-deoxy-glucose uptake, and increased acid-insoluble [³H]-thymidine incorporation, indicating renewed cellular DNA synthesis.
Infectious virus release dropped following serum-free arrest and failed to recover upon shifting to 35°C, whereas arrest in serum depleted of multiplication-stimulating factor preserved virus production.

Cite This Study

Bell et al. (1975) studied this question.

synapsesocial.com/papers/6a899e8b991508d05dc9913f https://doi.org/10.1099/0022-1317-27-2-127

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Implication

Experimental study reveals cell transformation and cycle entry in serum-starved fibroblasts after activating Rous sarcoma virus, indicating viral oncogenes drive proliferation without serum.

Transformation by a Temperature Sensitive Mutant of Rous Sarcoma Virus in the Absence of Serum

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Implication

Key Points

Cite This Study

Transformation by a Temperature Sensitive Mutant of Rous Sarcoma Virus in the Absence of Serum

Discussion

Member takes

Implication

Key Points

Cite This Study

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