September 1, 1999Molecular and Cellular BiologyOpen Access

In Vitro Import of a Nuclearly Encoded tRNA into the Mitochondrion of Trypanosoma brucei

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Population

Crude isolated Trypanosoma brucei mitochondria and synthetic RNAs transcribed from a cloned nucleus-encoded…

Comparison

In vitro import assay using a tandem tRNA… vs Mature-size tRNA(Leu)

Design

Preclinical

Authors

AYAudra E. Yermovsky-KammererUniversity of Alabama at Birmingham SHStephen L. HajdukUniversity of Georgia

Discussion

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Implication

Provides an in vitro system for trypanosome mitochondrial tRNA import; leaves open whether this pathway can be therapeutically targeted.

Structured PICO

Population

Crude isolated Trypanosoma brucei mitochondria and synthetic RNAs transcribed from a cloned nucleus-encoded tRNA gene cluster

Intervention

In vitro import assay using a tandem tRNA substrate composed of tRNA(Ser) and tRNA(Leu) transcribed in tandem with a 59-nucleotide intergenic region

Comparator

Mature-size tRNA(Leu)

Outcome

Import of tRNA into mitochondria and characterization of import requirements

This study establishes an in vitro system demonstrating that mitochondrial tRNA import in T. brucei prefers a tandemly linked precursor and requires specific protein, ATP, and membrane potential components.

Cite This Study

Yermovsky-Kammerer et al. (1999) studied this question.

synapsesocial.com/papers/6a89af255edafe343d92f051 https://doi.org/10.1128/mcb.19.9.6253

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In Vitro Import of a Nuclearly Encoded tRNA into the Mitochondrion of Trypanosoma brucei

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In Vitro Import of a Nuclearly Encoded tRNA into the Mitochondrion of Trypanosoma brucei

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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