Key result
A simple, validated RP-HPLC-UV method was successfully developed for the determination of azithromycin, demonstrating linearity over 1-80 µg/mL, high precision (RSD < 1.61%), and a detection limit of 0.3 µg/mL.
Why the study?
The study was conducted to develop and validate a new RP-HPLC method for estimation of ivabradine hydrochloride in pure and formulation, and a bioanalytical method for biological samples.
The study successfully developed and validated RP-HPLC and UV spectrophotometric methods for the accurate quantification of ivabradine hydrochloride in pharmaceutical formulations and rat plasma.
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Validated rat plasma methods aid preclinical ivabradine work; leaves open human bioanalytical translation.
Polker et al. (2020) studied this question. RP-HPLC-UV method vs. USP method was evaluated on Method validation parameters (linearity, precision, accuracy, sensitivity). A simple, validated RP-HPLC-UV method was successfully developed for the determination of azithromycin, demonstrating linearity over 1-80 µg/mL, high precision (RSD < 1.61%), and a detection limit of 0.3 µg/mL.
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