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September 1, 1982Journal of Biological ChemistryOpen Access

Role of Mg2+ in the Ca2+-Ca2+ exchange mediated by the membrane-bound (Ca2+, Mg2+)-ATPase of sarcoplasmic reticulum vesicles.

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Population

Sarcoplasmic reticulum vesicles preloaded with either 45Ca2+ or unlabeled Ca2+

Comparison

Elevation of Mg2+ concentration vs Low Mg2+ concentration (0.6 to 5.2 microM)

Design

Preclinical

Authors

YTYuichi TakakuwaTokyo Women's Medical UniversityTKT KanazawaKitami Institute of Technology

Discussion

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Implication

Mg2+ effects on SR Ca2+ exchange in vesicles may guide mechanistic studies; leaves open relevance to cardiac Ca2+ handling in vivo.

Structured PICO

P
Population
Sarcoplasmic reticulum vesicles preloaded with either 45Ca2+ or unlabeled Ca2+
I
Intervention
Elevation of Mg2+ concentration (5.2 microM to 4.8 mM)
C
Comparator
Low Mg2+ concentration (0.6 to 5.2 microM)
O
Outcome
Unidirectional Ca2+ efflux and influx, Ca2+-dependent ATP hydrolysis, and phosphorylation of the membrane-bound (Ca2+, Mg2+)-ATPasesurrogate

Mg2+ is essential for the exposure of the bound Ca2+ of the phosphoenzyme to the internal medium in sarcoplasmic reticulum vesicles.

Cite This Study

Takakuwa et al. (1982) studied this question.

synapsesocial.com/papers/6a89b5e8b1e5d05ea8ec81achttps://doi.org/10.1016/s0021-9258(18)33890-0
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Reaction mechanism of (Ca2+, Mg2+)-ATPase of sarcoplasmic reticulum vesicles. II. (ATP, ADP)-dependent Ca2+-Ca2+ exchange across the membranes.1981 · 23 citations
  2. 2Nonequivalent subunits in the calcium pump of sarcoplasmic reticulum.1981 · 91 citations
  3. 3The Role of Calcium and Magnesium in the Adenosine Triphosphatase Reaction of Sarcoplasmic Reticulum1971 · 76 citations
  4. 4Reaction mechanism of calcium-ATPase of sarcoplasmic reticulum. Substrates for phosphorylation reaction and back reaction, and further resolution of phosphorylated intermediates.1980 · 130 citations
  5. 5Formation and Decomposition of a Phosphorylated Intermediate in the Reaction of Na+-K+ Dependent ATPase*1970 · 149 citations