Reaction mechanism of (Ca2+, Mg2+)-ATPase of sarcoplasmic reticulum vesicles. II. (ATP, ADP)-dependent Ca2+-Ca2+ exchange across the membranes.

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Population

Sarcoplasmic reticulum vesicles preloaded with unlabeled CaCl2

Comparison

Addition of MgCl2 to external medium containing… vs Absence of added MgCl2

Design

Preclinical

Authors

Discussion

Hypothesis-generating for SR Ca2+ transport; extends biochemical models but leaves open cardiac relevance.

Population

Sarcoplasmic reticulum vesicles preloaded with unlabeled CaCl2

Intervention

Addition of MgCl2 to external medium containing 45CaCl2, ATP, and ADP

Comparator

Absence of added MgCl2

Outcome

Steady state rate of (ATP, ADP)-dependent 45Ca2+ incorporationsurrogate

MgCl2 addition markedly shifts the equilibrium of the reaction of Caout . EP and Cain . EP in sarcoplasmic reticulum vesicles.

Takakuwa et al. (1981) studied this question.

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