The study aimed to detect human coronaviruses in children with respiratory infections in Japan using molecular methods after failure to isolate them in standard cell cultures.
May aid detection of coronaviruses missed by culture in pediatric respiratory cases; leaves open diagnostic and clinical utility.
We have used the HHVe6MRG plate, containing HEF, HEp-2, VeroE6, MDCK, RD-18S, and GMK cell lines, for virus isolation to clarify the epidemiology of acute viral respiratory infections in children in Yamagata, Japan, since January 2004, as part of the National Epidemiological Surveillance of Infectious Diseases, Japan (NESID), based on the Infectious Diseases Control Law (1). We also prepared HMV-II and LLC-MK2 cell lines as separate 96-well tissue culture plates (Greiner Bio-One, Frickenhausen, Germany) since 2008 and 2011, respectively, to isolate parainfluenza viruses (2-4). However, we have not yet isolated any human coronaviruses (HCoVs), which are generally considered as common pathogens, using these systems. To confirm whether HCoVs circulate in Yamagata, we performed a study to detect HCoV-229E, -HKU1, -NL63, and -OC43 using a molecular method, i.e., reverse-transcription (RT)-PCR, and sequence analysis
No takes yet. Share an insight, caveat, or question.
Matoba et al. (2015) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: