What question did this study set out to answer?

To determine whether fast skeletal-type myosin light chains coexist with cardiac-type myosin light chains in embryonic chicken heart tissue.

Coexistence of Cardiac-Type and Fast Skeletal-Type Myosin Light Chains in Embryonic Chicken Cardiac Muscle1

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To determine whether fast skeletal-type myosin light chains coexist with cardiac-type myosin light chains in embryonic chicken heart tissue.
Analyzed embryonic chicken ventricular myosin using two-dimensional gel electrophoresis.
Performed immunoblot analysis using specific antibodies directed against fast skeletal myosin light chain 1 (anti-Lf1).
Mapped the subcellular localization of Lc1 and Lf1 antigens on myofibrils in embryonic cardiac muscle cells in vivo and in vitro.
Identified a minor light chain component in embryonic ventricular myosin that comigrated with fast skeletal myosin light chain 1 (Lf1) alongside cardiac light chains Lc1 and Lc2.
Confirmed specific reactivity of the minor light chain component with anti-Lf1 antibody via immunoblotting.
Detected both Lc1 and Lf1 antigens localized directly to myofibrils within embryonic cardiac muscle cells under both in vivo and in vitro conditions.

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Hypothesis-generating for developmental myosin isoform switching; leaves open relevance to human cardiogenesis or disease.

Obinata et al. (1983) studied this question.

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