Conditional Expression of a Truncated Fragment of Nonmuscle Myosin II-A Alters Cell Shape but Not Cytokinesis in HeLa Cells

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Population

HeLa cells (normally expressing only NMHC II-A and not NMHC II-B protein)

Comparison

Conditional expression of a truncated fragment… vs Expression of full-length human NMHC II-A or…

Design

Preclinical

Authors

Discussion

Delta N592 effects on cell morphology lack clinical relevance now; leaves open myosin II amino-terminus necessity for vertebrate cytokinesis.

Population

HeLa cells (normally expressing only NMHC II-A and not NMHC II-B protein)

Intervention

Conditional expression of a truncated fragment of nonmuscle myosin II-A heavy chain (delta N592) fused to GFP

Comparator

Expression of full-length human NMHC II-A or NMHC II-B fused to GFP, or repression of delta N592 by doxycycline

Outcome

Cell shape, actin filament rearrangement, focal adhesions, and localization to the cytokinetic ringsurrogate

The amino-terminus part of mammalian nonmuscle myosin II may be necessary for localization to the cytokinetic ring in vertebrates.

Wei et al. (2000) studied this question.

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Delta N592 effects on cell morphology lack clinical relevance now; leaves open myosin II amino-terminus necessity for vertebrate cytokinesis.