What question did this study set out to answer?

To characterize the cellular heterogeneity, functional phenotypes, and T-cell receptor repertoires of CMVpp65-reactivated CD4+ T cell subsets in human peripheral blood.

December 7, 2021Frontiers in ImmunologyOpen Access

Dissecting the Landscape of Activated CMV-Stimulated CD4+ T Cells in Humans by Linking Single-Cell RNA-Seq With T-Cell Receptor Sequencing

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To characterize the cellular heterogeneity, functional phenotypes, and T-cell receptor repertoires of CMVpp65-reactivated CD4+ T cell subsets in human peripheral blood.
Isolated CMVpp65-reactivated CD4+ T cells from human peripheral blood.
Conducted paired single-cell RNA sequencing and T-cell receptor (TCR) sequencing to evaluate transcriptomic profiles and clonal lineages across cell subsets.
Regulatory T cells (Tregs) represented the largest reactivated subset, displaying dual transcriptomic profiles associated with both inflammatory and inhibitory mechanisms.
Identified distinct cytotoxic CD4+ T subsets (CD4+ CTL1 and CD4+ CTL2) and recently activated CD4+ T cells (Tra), observing prominent clonal expansion and shared TCR repertoires between CTL1 and CTL2.

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Implication

Single-cell transcriptomic profiling reveals clonal expansion and phenotypic diversity of CMV-reactive CD4+ T cells in human blood, highlighting key mechanisms of antiviral immunity.

Cite This Study

Lyu et al. (2021) studied this question.

synapsesocial.com/papers/6a8a5fedaf65c378f7fd4daa https://doi.org/10.3389/fimmu.2021.779961

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Implication

Single-cell transcriptomic profiling reveals clonal expansion and phenotypic diversity of CMV-reactive CD4+ T cells in human blood, highlighting key mechanisms of antiviral immunity.

Dissecting the Landscape of Activated CMV-Stimulated CD4+ T Cells in Humans by Linking Single-Cell RNA-Seq With T-Cell Receptor Sequencing

Key Points

Discussion

Implication

Cite This Study

Dissecting the Landscape of Activated CMV-Stimulated CD4+ T Cells in Humans by Linking Single-Cell RNA-Seq With T-Cell Receptor Sequencing

Key Points

Discussion

Implication

Cite This Study

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