The implications of hyperoxia, type 1 diabetes and sex on cardiovascular physiology in mice

Figures

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  Figure 1. Akita male mice at both normoxia and hyperoxia experience a significant decrease in body and heart weight. (A) Body weight normalized to tibia length (g/cm) for all experimental groups, (B) Heart weight normalized to tibia length for all experimental groups (mg/cm), (C) H&E histological cross sections of Akita male and female mice under normoxia and hyperoxia, (D) Heart area cross sections (au), (E) WGA stained cardiac myocytes stained from Akita male and female mice. (F–I) Cardiac myocyte area of Akita male and female mice under normoxia or hyperoxia shown as follows: (F) LV cardiomyocte area, (G) RV cardiomyocte area, (H) Septum cardiomyocte area, (I) Pooled cardiomyocte area. Error bars represent ± SEM. *p < 0.05, **p < 0.005 ***p < 0.0005. *compares effects of hyperoxia and normoxia of same gender and strain; Y compares male and female mice of the same strain and treatment; ‡compares WT and Akita of same gender and treatment.

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  Figure 2. Akita mice display distinct functional parameters compared to WT mice (A) percent ejection fraction (FS), (B) percent fractional shortening (EF), (C) stroke volume (SV), and (D) cardiac output (CO) in hyperoxia/normoxia treated Akita and wild type mice, (E) Optical traces for male and female Akita mice in normoxic and hyperoxic conditions. Error bars represent ± SEM. *p < 0.05, **p < 0.005 ***p < 0.0005. *compares effects of hyperoxia and normoxia of same gender and strain; Y compares male and female mice of the same strain and treatment; ‡compares WT and Akita of same gender and treatment.

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  Figure 3. Hyperoxia induces bradyarrhythmias in Akita (and WT) mice (A) Original ECG traces in lead II mode in normoxia/hyperoxia treated Akita male and female mice, (B) RR interval, (C) PR interval, (D) QRS interval, (E) QTc interval, (F) JT interval. Error bars represent ± SEM. *p < 0.05, **p < 0.005 ***p < 0.0005. *compares effects of hyperoxia and normoxia of same gender and strain; Y compares male and female mice of the same strain and treatment; ‡compares WT and Akita of same gender and treatment.

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  Figure 4. Hyperoxia induce cardiotoxicity in Akita mice as evident by elevated levels of serum LDH in both sexes. Lactic Dehydrogenase (LDH) measured in serum from normoxia and hyperoxia WT and Akita groups. Error bars represent ± SEM. *p < 0.05, **p < 0.005 ***p < 0.0005. *compares effects of hyperoxia and normoxia of same gender and strain; Y compares male and female mice of the same strain and treatment.

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  Figure 5. Dysregulation of serum estradiol in Akita male mice in both normal air and hyperoxia. Estradiol levels were measured in serum from normoxia and hyperoxia WT and Akita groups using ELISA kit. Error bars represent ± SEM. *p < 0.05, **p < 0.005 ***p < 0.0005. *compares effects of hyperoxia and normoxia of same gender and strain; Y compares male and female mice of the same strain and treatment.