What question did this study set out to answer?

To determine whether the Na,K-ATPase, specifically its alpha-2 isozyme, mediates ouabain-induced cardiac inotropy in mice.

December 1, 2003Journal of Biological ChemistryOpen Access

The α2 Isoform of Na,K-ATPase Mediates Ouabain-induced Cardiac Inotropy in Mice

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Why the study?

Does the alpha2 isoform of Na,K-ATPase mediate ouabain-induced cardiac inotropy in mice?

Population

Mice expressing a ouabain-insensitive alpha2 isoform of the Na,K-ATPase and wild-type mice

Comparison

Administration of ouabain vs Wild-type mice

Design

Preclinical

Authors

IDIva DostanicYale University JLJohn N. LorenzHeart Failure / Cardiomyopathy JSJo El J. SchultzUniversity of Cincinnati Medical Center

Discussion

Member takes

Implication

Supports alpha2 mediation of ouabain inotropy in mice; leaves open human cardiac glycoside relevance.

Key Points

To determine whether the Na,K-ATPase, specifically its alpha-2 isozyme, mediates ouabain-induced cardiac inotropy in mice.
Generated homozygous knock-in mice expressing an engineered ouabain-insensitive alpha-2 isoform of Na,K-ATPase using Cre-Lox technology.
Assessed baseline cardiovascular function and measured cardiac contractility following ouabain administration in isolated hearts and in intact animals compared to wild-type controls.
Homozygous knock-in mice were born at expected Mendelian ratios, developed normally to adulthood, and maintained normal baseline cardiovascular function.
Ouabain administration stimulated cardiac inotropy in wild-type mouse hearts, but this inotropic response was completely absent in targeted knock-in mice expressing the ouabain-insensitive alpha-2 isoform.

Structured PICO

Does the alpha2 isoform of Na,K-ATPase mediate ouabain-induced cardiac inotropy in mice?

Population

Mice expressing a ouabain-insensitive alpha2 isoform of the Na,K-ATPase (developed using Cre-Lox technology) and wild-type mice

Intervention

Administration of ouabain

Comparator

Wild-type mice

Outcome

Cardiac contractility in isolated hearts and intact animalssurrogate

This study provides direct evidence that the alpha2 Na,K-ATPase isozyme specifically mediates ouabain-induced cardiac contractility in mice.

Cite This Study

Dostanic et al. (2003) studied this question.

synapsesocial.com/papers/6a8a79799be9d02581bdb4f0 https://doi.org/10.1074/jbc.m308547200

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The α2 Isoform of Na,K-ATPase Mediates Ouabain-induced Cardiac Inotropy in Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The α2 Isoform of Na,K-ATPase Mediates Ouabain-induced Cardiac Inotropy in Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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